Mike's Moss Resources

Physcomitrella links

Cosmoss genomics resources
Methods (Quatrano & Cove)
Methods (Reski)
Methods (Hasebe)
Phytozome (comparative plant genomics)

Mike's Moss Transformation Protocol
Mike's E. coli Ultra comp cell Protocol

ApE - A Plasmid Editor (for seq files)

pMP series vectors

Gateway Destination vectors for over-expression/inducible expression
pMP1155 (pUK-Pp108B-nptII-Ubi:Gateway:ocs)         Seq
pMP1228 (pUK-Pp108B-nptII-Ubix:Gateway:ocs)(no intron)    Seq
pMP1180 (pUK-Pp108B-nptII-GmHsp17.3B:Gateway:e9)    Seq
pMP1432 (pUK-Pp108B-Gent-GmHsp17.3B:Gateway:e9)    Seq


Moss selectable marker plasmids(derived from pBNRF)

pMP1109 (pBiLox+35S:nptII:camv) G418/Kanamycin-res.    Seq
pMP1159 (pBiLox+2x35S:Hyg:camv) Hygromycin-res.    Seq
pMP1169 (pBiLox+35S:Zeo:camv) Zeocin-res.    Seq
pMP1119 (pBiLox+35S:Gent:camv) Gentamycin-res.    Seq


Gateway Destination vectors for over-expression with tags

pMP1300 (pUK-Pp108B-nptII-Ubi:Gateway:GUS:ocs)    Seq
pMP1367 (pUK-Pp108B-nptII-Ubi:Gateway:TAP:ocs)    Seq
pMP1302 (pUK-Pp108B-nptII-Ubi:Gateway:TAP2:ocs)    Seq
pMP1298 (pUK-Pp108B-nptII-Ubi:Gateway:GFPhdel:ocs)    Seq
pMP1382 (pUK-Pp108B-nptII-Ubi:Gateway:GFP-His:ocs)    Seq
pMP1379 (pUK-Pp108B-nptII-Ubi:Gateway:YFP-HA:ocs)    Seq
pMP1574 (pUK-Pp108B-nptII-Ubi:Gateway:CFP-HA:ocs)    Seq
pMP1472 (pUK-Pp108B-nptII-Ubi:Gateway:YFPnls-HA:ocs)    Seq
pMP1442 (pUK-Pp108B-nptII-Ubi:Gateway:mVenus-HA:ocs)    Seq
pMP1474 (pUK-Pp108B-nptII-Ubi:Gateway:mVenusnls-HA:ocs)    Seq
pMP1445 (pUK-Pp108B-nptII-Ubi:Gateway:DsRED2-HA:ocs)    Seq
pMP1475 (pUK-Pp108B-nptII-Ubi:Gateway:DsRED2nls-HA:ocs)    Seq
pMP1377 (pUK-Pp108B-nptII-Ubi:Gateway:4xMyc:nos)    Seq
pMP1373 (pUK-Pp108B-nptII-Ubi:Gateway:1xMyc:ocs)    Seq
pMP1369 (pUK-Pp108B-nptII-Ubi:Gateway:HA:ocs)    Seq
pMP1371 (pUK-Pp108B-nptII-Ubi:Gateway:FLAG:ocs)    Seq
pMP1375 (pUK-Pp108B-nptII-Ubi:Gateway:AcV5:ocs)    Seq
pMP1575 (pUK-Pp108B-nptII-Ubi:Gateway:GRlbd:ocs)    Seq
pMP1578 (pUK-Pp108B-nptII-Ubi:Gateway:ESR1lbd:ocs)    Seq
pMP1335 (pUK-Pp108B-nptII-Ubi:GFP6:Gateway:ocs)    Seq
pMP1361 (pUK-Pp108B-nptII-Ubi: YFP:Gateway:ocs)    Seq
pMP1363 (pUK-Pp108B-nptII-Ubi:TAP2:Gateway:ocs)    Seq
pMP1359 (pUK-Pp108B-nptII-Ubi:1xMyc:Gateway:ocs)    Seq
pMP1355 (pUK-Pp108B-nptII-Ubi:HA:Gateway:ocs)    Seq
pMP1357 (pUK-Pp108B-nptII-Ubi:FLAG:Gateway:ocs)    Seq
pMP1365 (pUK-Pp108B-nptII-Ubi:AcV5:Gateway:ocs)    Seq


Gateway Destination vectors for RNAi (no GUS/GFP co-silencing)

pMP1331 (pBiLoxHyg+Ubix:Hellsgate12:ocs)    Seq
pMP1333 (pUK-Pp108B-Hyg+Ubix:Hellsgate12:ocs)    Seq
pMP882 (pENTR-NotIBamHISmaIEcoRI)    Seq


Physcomitrella neutral target loci plasmids

pMP1141 (pGEM+Pp108A) hydrolase pseudogene target    Seq
pMP1033 (pUK+Pp108B) adjacent intergenic region target    Seq
Endogenous Pp108 target locus    Seq