LOCUS                pUK_Pp108        5713 bp ds-DNA   circular     28-OCT-2008
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     gggccacgaaggccnnnggccgatctggcc
            ggtggccagatcGGCCATGTGccacacatnnnggccttcgtggcc
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             3983..4801
                     /label=KanR
                     /ApEinfo_fwdcolor=#ffff66
                     /ApEinfo_revcolor=#ffff66
     misc_feature    join(60..3105,3107..3114)
                     /label=Pp108
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
     rep_origin      4868..5550
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             3118..3186
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_revcolor=cornflower blue
     misc_feature    complement(3171..3187)
                     /label=pUK-PvR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    join(5708..5713,1..11)
                     /label=pUK-PvF
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    3117..3119
                     /label=PvuII
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    53..55
                     /label=PvuII(1)
                     /ApEinfo_label=PvuII
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagggcca
       61 cgaaggccgt tcttccctgg gtgcaggcta ctcagtcgga cttgggtatt cttcttgtca
      121 tataacctgc ttctcgattt gtagactaga gaccgtagat tgattcgtgc ccttggtata
      181 tggctatttg ccagagagat taactcatcg atttggcttt tctaatgtat ctatgttcat
      241 tacacgtgag aagcccgttc tttccctttg attattattg taatgttttg cctttctgtg
      301 tccatgttga ttaggttcct aacttccaga gaatcgagta atgtgaataa cctgcacagt
      361 acacacttca catcccgttt catgggaagt ttttaaaacg atactgaaca ctaacttttg
      421 tagccatttt gatgtaattt tagtaatgta taatttgaaa ttggttcatg ttactttctg
      481 tctttaaaat gttcgtagca tgtgttgatt aatttgtgta agacagatgg atcattgtgt
      541 ctattaatac aaattaaaca aaactatttg tccataacag atctttggaa taacaccaaa
      601 cactactgca agattgcaac acactgagtt taatatcttc taattgattt ccttgtgcat
      661 gtttgtgctg aattattgtg tgtagtactt tgtagtgctt tgattgcata tgtcaatcaa
      721 tttataatcg ataatcaata ttaagggtac caaatacaaa aacagataaa actactaaaa
      781 cgttgggatt gaatgttcaa cacgatcaat gcttgtgtaa tgttcacatg gcacaaatcg
      841 atagatattt taaagaattt agcaacaatt ttttctagtg acaagtggat taattacata
      901 ttcttcaaaa cccatagata ttacaatacc aataaaccac aaatattaat aaaaatataa
      961 tacttgaatt tcatatactt tattaataaa cattaacata caaattagca attagacaaa
     1021 accatttatg aacaagaatg caattctttt gtgaatctta tgactataac caacaaatgg
     1081 ttttgaaaat caaatcttaa aatcaattta tataattaac aaatattttt agatgagaaa
     1141 ctattttatg ttctattatt gataatataa tatttttcat gatctaaatg taaactttta
     1201 tatttaataa atataatcaa aacacaataa aattgcttca attattatat cattgagaaa
     1261 cttgcttcac atcatacttg gttttgaatg atatatcata tacatgggtg tgggattcaa
     1321 agctcaaata aatttctttt agttaaaata ttaataaaga tttggtcata tcccatgcta
     1381 agataatata tacctcaaaa agaaagaatt actagtaaaa gcacataagg tagccaattc
     1441 attatcaacc acctcccatt tccaacatat agtcaattag ttctgatata ttatggttgg
     1501 aaacaggcct agcagaaatg atattaaatt gtgagtaata taaacaattc attgtactca
     1561 gaattagtag gcgtgaattg tcaaaaatta atttttgtat tagtagaatt ccacatgtgc
     1621 tacaattgat tatgcatcaa caaatcaagg agtcattgga gtaagattgg aagatcgagg
     1681 atgtataaaa tattgtttgg agatgagaag aaatgaggaa gaatagtggc aaaaatccaa
     1741 ctcaaacatg gtggattact cacaactgga tgtttgaatt tttgatgtct catccacata
     1801 tttacatatg tatgtagcat ggaattaggt gaggtttgat aggataatta ggtgccatac
     1861 atcacagttt tgtaaaccac cactagtcag aattagattg acatatatgt tgaaaatgga
     1921 tcaaagagaa tcttcagcta aaaaaattat ataaattcta tttttggacc atattgtata
     1981 aaaacacttg aaatcctctt gatttctatt tccctacatg ggggtatttt tctattttta
     2041 caactattca atacatacta aaatgctgaa gttattggaa atgggttgtc aaccttgtat
     2101 tacatccacg atggatactt agtgtagagt tagagatctc ttacttgatc atatatatat
     2161 gtatatgtaa atgtgtttca acaaaacgag taccattaaa gatgtttctg acttggatta
     2221 agtgctaatt ccattacagt tgatactgtt acatgtcaac ttccaattac atgtggacga
     2281 acatgtttga cggaaaatgt ccacactcac ctagatttca ttagtcatcc attggtcatc
     2341 taatctgaaa cgtccacagc attagtcgaa agacggaagg aatcaacgcc gacaacaaga
     2401 aaccaggtgc gagatcgagg ggtgagggcg gctgcatagg tgtcggtagt gcactccgtt
     2461 cgtagagttt agagtttctc tgcattgttg tcaaaaaaca gcgagcctct tgcaaatgga
     2521 ggtggtgaaa tcaatgctgt acacagaagg agacgatcgg ttgcctctct attggtacat
     2581 cgacaccgtg gtgattgcat tgcgaagaca agtctagggt gcatgtgaga gacactgggc
     2641 aagctggagg cgacaagcgt gaggtattca cgctcgtgga gtcgaactgg atgcaatcgg
     2701 ggatttacaa gcacagcttg acagtcaaga cggcgggtat gaagtttctg atgcaaaatt
     2761 cgtcgccacg tctagatcat acgcagcatg tgcggactgt ggagcatcct gtacgattgc
     2821 gactggatgt taatattttc tttttccagc caggaaagag taaactcatc tgaccaagaa
     2881 tggaatgccg aaatcgtcac agtcgagcat ttgcggagaa agaaccctcc ttgcgaatgt
     2941 gttgtccctt gttgaaatga atcgtactgc tgagtcatga cgatatacaa gagcatccaa
     3001 ctgcttagat ctgacaggaa gagtaattag gtgtggggtt attgtggact tagacatttg
     3061 tctcaagttc tagtctcgca tcacggaagc cggagaagga tgtggccgat ctggccctgg
     3121 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     3181 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3241 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3301 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3361 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggtgggc
     3421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     3481 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     3541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     3601 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     3661 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     3721 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     3781 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     3841 tacgcctatt tttataggtt aatgtcatgg gggggggcac gttgtgtctc aaaatctctg
     3901 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     3961 aaacagtaat acaaggggtg ttATGAGCCA TATTCAACGG GAAACGTCTT GCTCGACGAG
     4021 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA
     4081 TGTCGGGCAA TCAGGTGCGA CAATCTACCG ATTGTATGGG AAGCCCGATG CGCCAGAGTT
     4141 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT
     4201 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA
     4261 TGATGCATGG TTACTCACCA CTGCGATCCC CGGCAAAACA GCATTCCAGG TATTAGAAGA
     4321 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA
     4381 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC
     4441 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG
     4501 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAGCTc TTGCCATTCT CACCGGATTC
     4561 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT
     4621 AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT
     4681 ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG
     4741 TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA
     4801 Atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     4861 cgcaagctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4921 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4981 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5041 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     5101 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5161 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5221 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5281 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     5341 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5401 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5461 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5521 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5581 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5641 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5701 gaggaagcgg aag    
//
