LOCUS       pUK_Pp108_UbixGW_n       10152 bp ds-DNA   circular     02-DEC-2009
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     gggccacgaaggccnnnggccgatctggcc
            ggtggccagatcGGCCATGTGccacacatnnnggccttcgtggcc
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             8422..9240
                     /label=KanR
                     /ApEinfo_fwdcolor=#ffff66
                     /ApEinfo_revcolor=#ffff66
     misc_feature    60..1411
                     /label=Pp108
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
     primer_bind     4770..4790
                     /label=Ubipr
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     CDS             complement(2985..3641)
                     /label=CmR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     rep_origin      9307..9989
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     promoter        4808..5198
                     /note="Cauliflower Mosaic Virus 35S promoter"
                     /label=Cauliflower Mosaic Virus 35S promoter
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
     promoter        complement(3888..4794)
                     /label=ZmUbi PromIntron
                     /ApEinfo_fwdcolor=#ffcc66
                     /ApEinfo_revcolor=#ffcc66
     misc_recomb     complement(6287..6320)
                     /label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
     misc_feature    2171..2191
                     /label=AttB2
                     /ApEinfo_fwdcolor=#dda0dd
                     /ApEinfo_revcolor=#dda0dd
     CDS             7557..7625
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_revcolor=cornflower blue
     misc_feature    2126..2145
                     /label=OCS-R
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    complement(7610..7626)
                     /label=pUK-PvR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    complement(3853..3873)
                     /label=AttB1
                     /ApEinfo_fwdcolor=#dda0dd
                     /ApEinfo_revcolor=#dda0dd
     terminator      6051..6255
                     /label=Tcamv
                     /ApEinfo_fwdcolor=#800000
                     /ApEinfo_revcolor=#800000
     misc_feature    complement(3860..3866)
                     /label=Gateway recomb1
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     misc_feature    complement(1587..2155)
                     /label=OCS term
                     /ApEinfo_fwdcolor=#ff6666
                     /ApEinfo_revcolor=#ff6666
     misc_feature    join(10147..10152,1..11)
                     /label=pUK-PvF
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    7556..7558
                     /label=PvuII
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     3920..3938
                     /label=Ubi-992R
                     /ApEinfo_fwdcolor=#66ccff
                     /ApEinfo_revcolor=green
     misc_feature    53..55
                     /label=PvuII(1)
                     /ApEinfo_label=PvuII
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     gene            5231..6025
                     /gene="nptII"
                     /label=nptII
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    join(6326..7544,7546..7553)
                     /label=Pp108(1)
                     /ApEinfo_label=Pp108
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
     CDS             complement(2338..2547)
                     /label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     misc_feature    2178..2184
                     /label=Gateway Recomb2
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     primer_bind     5149..5168
                     /label=35Stata-F
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagggcca
       61 cgaaggccgt tcttccctgg gtgcaggcta ctcagtcgga cttgggtatt cttcttgtca
      121 tataacctgc ttctcgattt gtagactaga gaccgtagat tgattcgtgc ccttggtata
      181 tggctatttg ccagagagat taactcatcg atttggcttt tctaatgtat ctatgttcat
      241 tacacgtgag aagcccgttc tttccctttg attattattg taatgttttg cctttctgtg
      301 tccatgttga ttaggttcct aacttccaga gaatcgagta atgtgaataa cctgcacagt
      361 acacacttca catcccgttt catgggaagt ttttaaaacg atactgaaca ctaacttttg
      421 tagccatttt gatgtaattt tagtaatgta taatttgaaa ttggttcatg ttactttctg
      481 tctttaaaat gttcgtagca tgtgttgatt aatttgtgta agacagatgg atcattgtgt
      541 ctattaatac aaattaaaca aaactatttg tccataacag atctttggaa taacaccaaa
      601 cactactgca agattgcaac acactgagtt taatatcttc taattgattt ccttgtgcat
      661 gtttgtgctg aattattgtg tgtagtactt tgtagtgctt tgattgcata tgtcaatcaa
      721 tttataatcg ataatcaata ttaagggtac caaatacaaa aacagataaa actactaaaa
      781 cgttgggatt gaatgttcaa cacgatcaat gcttgtgtaa tgttcacatg gcacaaatcg
      841 atagatattt taaagaattt agcaacaatt ttttctagtg acaagtggat taattacata
      901 ttcttcaaaa cccatagata ttacaatacc aataaaccac aaatattaat aaaaatataa
      961 tacttgaatt tcatatactt tattaataaa cattaacata caaattagca attagacaaa
     1021 accatttatg aacaagaatg caattctttt gtgaatctta tgactataac caacaaatgg
     1081 ttttgaaaat caaatcttaa aatcaattta tataattaac aaatattttt agatgagaaa
     1141 ctattttatg ttctattatt gataatataa tatttttcat gatctaaatg taaactttta
     1201 tatttaataa atataatcaa aacacaataa aattgcttca attattatat cattgagaaa
     1261 cttgcttcac atcatacttg gttttgaatg atatatcata tacatgggtg tgggattcaa
     1321 agctcaaata aatttctttt agttaaaata ttaataaaga tttggtcata tcccatgcta
     1381 agataatata tacctcaaaa agaaagaatt actaggcgga ccgaagcttg catgcctgca
     1441 ggtcctgctg agcctcgaca tgttgtcgca aaattcgccc tggacccgcc caacgatttg
     1501 tcgtcactgt caaggtttga cctgcacttc atttggggcc cacatacacc aaaaaaatgc
     1561 tgcataattc tcggggcagc aagtcggtta cccggccgcc gtgctggacc gggttgaatg
     1621 gtgcccgtaa ctttcggtag agcggacggc caatactcaa cttcaaggaa tctcacccat
     1681 gcgcgccggc ggggaaccgg agttcccttc agtgagcgtt attagttcgc cgctcggtgt
     1741 gtcgtagata ctagcccctg gggcactttt gaaatttgaa taagatttat gtaatcagtc
     1801 ttttaggttt gaccggttct gccgcttttt ttaaaattgg atttgtaata ataaaacgca
     1861 attgtttgtt attgtggcgc tctatcatag atgtcgctat aaacctattc agcacaatat
     1921 attgttttca ttttaatatt gtacatataa gtagtagggt acaatcagta aattgaacgg
     1981 agaatattat tcataaaaat acgatagtaa cgggtgatat attcattaga atgaaccgaa
     2041 accggcggta aggatctgag ctacacatgc tcaggttttt tacaacgtgc acaacagaat
     2101 tgaaagcaaa tatcatgcga tcataggcgt ctcgcatatc tcattaaagc aggactctag
     2161 ggactagGCA CCACTTTGTA CAAGAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC
     2221 AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT AATGCTGTAA AACACAACAT
     2281 ATCCAGTCAC TATGGTCGAC CTGCAGACTG GCTGTGTATA AGGGAGCCTG ACATTTATAT
     2341 TCCCCAGAAC ATCAGGTTAA TGGCGTTTTT GATGTCATTT TCGCGGTGGC TGAGATCAGC
     2401 CACTTCTTCC CCGATAACGG AGACCGGCAC ACTGGCCATA TCGGTGGTCA TCATGCGCCA
     2461 GCTTTCATCC CCGATATGCA CCACCGGGTA AAGTTCACGG GAGACTTTAT CTGACAGCAG
     2521 ACGTGCACTG GCCAGGGGGA TCACCATCCG TCGCCCGGGC GTGTCAATAA TATCACTCTG
     2581 TACATCCACA AACAGACGAT AACGGCTCTC TCTTTTATAG GTGTAAACCT TAAACTGCAT
     2641 TTCACCAGCC CCTGTTCTCG TCAGCAAAAG AGCCGTTCAT TTCAATAAAC CGGGCGACCT
     2701 CAGCCATCCC TTCCTGATTT TCCGCTTTCC AGCGTTCGGC ACGCAGACGA CGGGCTTCAT
     2761 TCTGCATGGT TGTGCTTACC AGACCGGAGA TATTGACATC ATATATGCCT TGAGCAACTG
     2821 ATAGCTGTCG CTGTCAACTG TCACTGTAAT ACGCTGCTTC ATAGCATACC TCTTTTTGAC
     2881 ATACTTCGGG TATACATATC AGTATATATT CTTATACCGC AAAAATCAGC GCGCAAATAC
     2941 GCATACTGTT ATCTGGCTTT TAGTAAGCCG GATCCACGCG TTTACGCCCC GCCCTGCCAC
     3001 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAACG
     3061 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG
     3121 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG
     3181 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG
     3241 AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC
     3301 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA
     3361 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA
     3421 CGGAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC
     3481 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG
     3541 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG
     3601 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT
     3661 GAAAATCTCG ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT
     3721 GTAAAGCCTG GGGTGCCTAA TGCGGCCGCC AATATGACTG GATATGTTGT GTTTTACAGT
     3781 ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT
     3841 ACGTTTCTCG TTCAGCTTTT TTGTACAAAC TTGTGATctc gacTCTAGGA TCTGGTTGTG
     3901 TGTGTGTGCG CTCCGAACAA CACGAGGTTG GGGAAagagg gtgtggaggg ggtgtctatt
     3961 tattacggcg ggcgaggaag ggaaagcgaa ggagcggtgg gaaaggaatc ccccgtagct
     4021 gccgtgccgt gagaggagga ggaggccgcc tgccgtgccg gctcacgtct gccgctccgc
     4081 cacgcaattt ctggatgccg acagcggagc aagtccaacg gtggagcgga actctcgaga
     4141 ggggtccaga ggcagcgaca gagatgccgt gccgtctgct tcgcttggcc cgacgcgacg
     4201 ctgctggttc gctggttggt gtccgttaga ctcgtcgacg gcgtttaaca ggctggcatt
     4261 atctactcga aacaagaaaa atgtttcctt agttttttta atttcttaaa gggtatttgt
     4321 ttaattttta gtcactttat tttattctat tttatatcta aattattaaa taaaaaaact
     4381 aaaatagagt tttagttttc ttaatttaga ggctaaaata gaataaaata gatgtactaa
     4441 aaaaattagt ctataaaaac cattaaccct aaaccctaaa tggatgtact aataaaatgg
     4501 atgaagtatt atataggtga agctatttgc aaaaaaaaag gagaacacat gcacactaaa
     4561 aagataaaac tgtagagtcc tgttgtcaaa atactcaatt gtcctttaga ccatgtctaa
     4621 ctgttcattt atatgattct ctaaaacact gatattattg tagtactata gattatatta
     4681 ttcgtagagt aaagtttaaa tatatgtata aagatagata aactgcactt caaacaagtg
     4741 tgacaaaaaa aatatgtggt aattttttat aacttagaca tgcaatgctc attaTCTCTA
     4801 GGtACcccta ctccaaaaat gtcaaagata cagtctcaga agaccaaagg gctattgaga
     4861 cttttcaaca aagggtaatt tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     4921 acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat cattgcgata
     4981 aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     5041 ccncgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     5101 gatgtgacat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     5161 cttcctctat ataaggaagt tcatttcatt tggagaggac agcccaagca tggagaaccg
     5221 acctgcaggc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5281 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5341 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5401 gaatgaactc caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5461 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5521 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5581 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5641 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5701 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5761 gatgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5821 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5881 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5941 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6001 tcgccttctt gacgagttct tctgagcggg actctggggt tcggacggta cgctgaaatc
     6061 accagtctct tctacaaatc tatctctctc tattttctcc ataaataatg tgtgagtagt
     6121 ttcccgataa gggaaattag ggttcttata gggtttcgct catgtgttga gcatataaga
     6181 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     6241 aaaccaaaat ccagtactaa aatccagatc AATTCgcggc cGATCTATAA CTTCGTATAA
     6301 TGTATGCTAT ACGAAGTTAT ACTAGtcaga attagattga catatatgtt gaaaatggat
     6361 caaagagaat cttcagctaa aaaaattata taaattctat ttttggacca tattgtataa
     6421 aaacacttga aatcctcttg atttctattt ccctacatgg gggtattttt ctatttttac
     6481 aactattcaa tacatactaa aatgctgaag ttattggaaa tgggttgtca accttgtatt
     6541 acatccacga tggatactta gtgtagagtt agagatctct tacttgatca tatatatatg
     6601 tatatgtaaa tgtgtttcaa caaaacgagt accattaaag atgtttctga cttggattaa
     6661 gtgctaattc cattacagtt gatactgtta catgtcaact tccaattaca tgtggacgaa
     6721 catgtttgac ggaaaatgtc cacactcacc tagatttcat tagtcatcca ttggtcatct
     6781 aatctgaaac gtccacagca ttagtcgaaa gacggaagga atcaacgccg acaacaagaa
     6841 accaggtgcg agatcgaggg gtgagggcgg ctgcataggt gtcggtagtg cactccgttc
     6901 gtagagttta gagtttctct gcattgttgt caaaaaacag cgagcctctt gcaaatggag
     6961 gtggtgaaat caatgctgta cacagaagga gacgatcggt tgcctctcta ttggtacatc
     7021 gacaccgtgg tgattgcatt gcgaagacaa gtctagggtg catgtgagag acactgggca
     7081 agctggaggc gacaagcgtg aggtattcac gctcgtggag tcgaactgga tgcaatcggg
     7141 gatttacaag cacagcttga cagtcaagac ggcgggtatg aagtttctga tgcaaaattc
     7201 gtcgccacgt ctagatcata cgcagcatgt gcggactgtg gagcatcctg tacgattgcg
     7261 actggatgtt aatattttct ttttccagcc aggaaagagt aaactcatct gaccaagaat
     7321 ggaatgccga aatcgtcaca gtcgagcatt tgcggagaaa gaaccctcct tgcgaatgtg
     7381 ttgtcccttg ttgaaatgaa tcgtactgct gagtcatgac gatatacaag agcatccaac
     7441 tgcttagatc tgacaggaag agtaattagg tgtggggtta ttgtggactt agacatttgt
     7501 ctcaagttct agtctcgcat cacggaagcc ggagaaggat gtggccgatc tggccctggc
     7561 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     7621 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     7681 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7741 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7801 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggtgggcc
     7861 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     7921 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
     7981 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     8041 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     8101 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     8161 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     8221 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     8281 acgcctattt ttataggtta atgtcatggg ggggggcacg ttgtgtctca aaatctctga
     8341 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     8401 aacagtaata caaggggtgt tATGAGCCAT ATTCAACGGG AAACGTCTTG CTCGACGAGG
     8461 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT
     8521 GTCGGGCAAT CAGGTGCGAC AATCTACCGA TTGTATGGGA AGCCCGATGC GCCAGAGTTG
     8581 TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA
     8641 AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT
     8701 GATGCATGGT TACTCACCAC TGCGATCCCC GGCAAAACAG CATTCCAGGT ATTAGAAGAA
     8761 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT
     8821 TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG
     8881 CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC
     8941 TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAGCTcT TGCCATTCTC ACCGGATTCA
     9001 GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA
     9061 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA
     9121 TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT
     9181 ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA
     9241 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     9301 gcaagctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9361 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9421 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9481 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     9541 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9601 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9661 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9721 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     9781 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9841 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9901 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9961 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    10021 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    10081 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    10141 aggaagcgga ag    
//
