LOCUS               4113 bp ds-DNA     circular     09-NOV-2007
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     lox-35S-hygro-cter-lox prim (a) fw:m13F prim (b):m13rev
            Hyg has false ATG and no stop!!!
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     141..158
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     terminator      join(1183..1248,1250..1341)
                     /label=Tcamv
                     /ApEinfo_fwdcolor=#ff6666
                     /ApEinfo_revcolor=#ff6666
     CDS             800..1174
                     /label=Zeocin
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#cc66ff
     primer_bind     complement(1501..1521)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     promoter        join(322..698,700..779)
                     /label=35S
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
     primer_bind     complement(1471..1490)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     rep_origin      complement(1889..2571)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     primer_bind     322..339
                     /label=P35S-KpnF
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     CDS             complement(2..70)
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_revcolor=cornflower blue
     primer_bind     complement(1317..1341)
                     /label=T35S-SacI
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     CDS             complement(2669..3328)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     misc_recomb     complement(282..315)
                     /label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
     misc_recomb     complement(1365..1398)
                     /label=LoxP(1)
                     /ApEinfo_label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
ORIGIN
        1 CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC
       61 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG
      121 GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGC Caagctaatt accctgttat
      181 ccctaggcgg accgaagctt gcatgcctgc aggtcgactc tagaggatcc cacgtgcccg
      241 ggcgtttaaa ccaattgttc gaacgtacgt cgcgaCTCGA GATAACTTCG TATAATGTAT
      301 GCTATACGAA GTTATggtac caacatggtg gagcacgaca ctctcgtcta ctccaagaat
      361 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
      481 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
      721 tcatttcatt tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc
      781 tcgaGTGAGG AGGGCCACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG
      841 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT
      901 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA
      961 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT
     1021 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT
     1081 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA
     1141 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACtcg agtttctcca taataatgtg
     1201 tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcgt gtgttgagca
     1261 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     1321 attcctaaaa ccaaaatcca gGaGctCGAA TTCgcggccG ATCTATAACT TCGTATAATG
     1381 TATGCTATAC GAAGTTATAC TAGTggcgcc acgcgtgata tcatgcatgt taacatcgat
     1441 ccatgggcgc gccttaatta aatttaaatt CCCTATAGTG AGTCGTATTA AATTCGTAAT
     1501 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC
     1561 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA
     1621 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT
     1681 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC
     1741 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG
     1801 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG
     1861 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC
     1921 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG
     1981 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA
     2041 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC
     2101 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG
     2161 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT
     2221 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA
     2281 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA
     2341 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG
     2401 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA
     2461 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG
     2521 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA
     2581 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA
     2641 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG
     2701 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA
     2761 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC
     2821 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC
     2881 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA
     2941 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC
     3001 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT
     3061 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA
     3121 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG
     3181 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG
     3241 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC
     3301 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT
     3361 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT
     3421 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG
     3481 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC
     3541 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA
     3601 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG
     3661 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA
     3721 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT
     3781 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGCATCCC TTTAGGGTTC CGATTTAGTG
     3841 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT
     3901 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC
     3961 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG
     4021 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG
     4081 CGAATTTTAA CAAAATATTA ACGTTTACAA TTT  
//
