LOCUS           pUK_Hsp_GW_108        9657 bp ds-DNA   circular     04-JUL-2008
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     gggccacgaaggccnnnggccgatctggcc
            ggtggccagatcGGCCATGTGccacacatnnnggccttcgtggcc
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             7927..8745
                     /label=KanR
                     /ApEinfo_fwdcolor=#ffff66
                     /ApEinfo_revcolor=#ffff66
     misc_feature    join(5831..7049,7051..7058,60..1411)
                     /label=Pp108B
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
     promoter        4312..4702
                     /note="Cauliflower Mosaic Virus 35S promoter"
                     /label=CaMV 35S promoter
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
     promoter        1454..1767
                     /vntifkey="29"
                     /label=GmHsp17.3B promoter
                     /ApEinfo_fwdcolor=seashell4
                     /ApEinfo_revcolor=seashell4
     CDS             2060..2716
                     /label=CmR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     misc_feature    complement(3510..3530)
                     /label=AttB2
                     /ApEinfo_fwdcolor=#dda0dd
                     /ApEinfo_revcolor=#dda0dd
     misc_feature    1828..1848
                     /label=AttB1
                     /ApEinfo_fwdcolor=#dda0dd
                     /ApEinfo_revcolor=#dda0dd
     CDS             join(3154..3198,3200..3363)
                     /label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     primer_bind     complement(5499..5520)
                     /label=KANF
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     rep_origin      8812..9494
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             7062..7130
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_revcolor=cornflower blue
     gene            4735..5529
                     /gene="nptII"
                     /label=nptII
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     terminator      3631..4248
                     /label=E9
                     /ApEinfo_fwdcolor=#ff6666
                     /ApEinfo_revcolor=#ff6666
     misc_recomb     complement(4274..4307)
                     /label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
     misc_recomb     complement(5792..5825)
                     /label=LoxP(1)
                     /ApEinfo_label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
     primer_bind     4653..4672
                     /label=35Stata-F
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     4736..4757
                     /label=KANR
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     misc_feature    complement(7115..7131)
                     /label=pUK-PvR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     terminator      join(5599..5607,5609..5759)
                     /label=Tcamv
                     /ApEinfo_fwdcolor=#800000
                     /ApEinfo_revcolor=#800000
     misc_feature    join(9652..9657,1..11)
                     /label=pUK-PvF
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     repeat_region   complement(3407..3531)
                     /vntifkey="34"
                     /label=attR2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     repeat_region   complement(1822..1951)
                     /vntifkey="34"
                     /label=attR1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagggcca
       61 cgaaggccgt tcttccctgg gtgcaggcta ctcagtcgga cttgggtatt cttcttgtca
      121 tataacctgc ttctcgattt gtagactaga gaccgtagat tgattcgtgc ccttggtata
      181 tggctatttg ccagagagat taactcatcg atttggcttt tctaatgtat ctatgttcat
      241 tacacgtgag aagcccgttc tttccctttg attattattg taatgttttg cctttctgtg
      301 tccatgttga ttaggttcct aacttccaga gaatcgagta atgtgaataa cctgcacagt
      361 acacacttca catcccgttt catgggaagt ttttaaaacg atactgaaca ctaacttttg
      421 tagccatttt gatgtaattt tagtaatgta taatttgaaa ttggttcatg ttactttctg
      481 tctttaaaat gttcgtagca tgtgttgatt aatttgtgta agacagatgg atcattgtgt
      541 ctattaatac aaattaaaca aaactatttg tccataacag atctttggaa taacaccaaa
      601 cactactgca agattgcaac acactgagtt taatatcttc taattgattt ccttgtgcat
      661 gtttgtgctg aattattgtg tgtagtactt tgtagtgctt tgattgcata tgtcaatcaa
      721 tttataatcg ataatcaata ttaagggtac caaatacaaa aacagataaa actactaaaa
      781 cgttgggatt gaatgttcaa cacgatcaat gcttgtgtaa tgttcacatg gcacaaatcg
      841 atagatattt taaagaattt agcaacaatt ttttctagtg acaagtggat taattacata
      901 ttcttcaaaa cccatagata ttacaatacc aataaaccac aaatattaat aaaaatataa
      961 tacttgaatt tcatatactt tattaataaa cattaacata caaattagca attagacaaa
     1021 accatttatg aacaagaatg caattctttt gtgaatctta tgactataac caacaaatgg
     1081 ttttgaaaat caaatcttaa aatcaattta tataattaac aaatattttt agatgagaaa
     1141 ctattttatg ttctattatt gataatataa tatttttcat gatctaaatg taaactttta
     1201 tatttaataa atataatcaa aacacaataa aattgcttca attattatat cattgagaaa
     1261 cttgcttcac atcatacttg gttttgaatg atatatcata tacatgggtg tgggattcaa
     1321 agctcaaata aatttctttt agttaaaata ttaataaaga tttggtcata tcccatgcta
     1381 agataatata tacctcaaaa agaaagaatt actaggcgga ccgaagcttg catgcctgca
     1441 ggtcgacgga tcccccctgg tagaccaatc ctaaccaatg tctggttaag atggtccaat
     1501 cccgaaactt ctagttgcgg ttcgaagaag tccagaatgt ttctgaaagt ttcagaaaat
     1561 tctagttttg agattttcag aagtacggca tgatgatgca taacaaggac tttctcgaaa
     1621 gtactatatt gctcctctac atcattttaa ataccccatg tgtcctttga agacacatca
     1681 cagaaagaag tgaaggcatc gttagcagtt ttgtagattc aacctcaatt tgcagagtta
     1741 cgttctaata tatttacaca agactgggga tcctctagag gatccccggg taccgggccc
     1801 cccctcgagg cgcgccaagc tatcaaacaa gtttgtacaa aaaagctgaa cgagaaacgt
     1861 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat
     1921 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac
     1981 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc
     2041 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     2101 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     2161 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     2221 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
     2281 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     2341 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     2401 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     2461 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     2521 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     2581 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     2641 cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     2701 gtggcagggc ggggcgtaat ctagaggatc cggcttacta aaagccagat aacagtatgc
     2761 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat
     2821 gtcaaaaaga ggtAtgctat gaagcagcgt attacagtga cagttgacag cgacagctat
     2881 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga
     2941 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
     3001 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg gGctggtgaa
     3061 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
     3121 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
     3181 ctgctgtcag ataaagtcCc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
     3241 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
     3301 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
     3361 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
     3421 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
     3481 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg ttcgataatt
     3541 cttaattaac tagaggatcc cacgggctgc aggaattcga tAGCTTatcg ataccgtcga
     3601 cctcgacctg taccttctag tannnnnTTT TCGTTCGTAT CATCGGTTTC GACAACGTTC
     3661 GTCAAGTTCA ATGCATCAGT TTCATTGCGC ACACACCAGA ATCCTACTGA gttcgagtat
     3721 tatggcattg ggaaacatgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt
     3781 ttttattcgg ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa
     3841 tgatatggtc cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg
     3901 tgtgttgaat ttgaaaatat aagagatatg caaacatttt gttttgagta aaaatgtgtc
     3961 aaatcgtggc ctctaatgac cgaagttaat atgaggagta aaacacttgt agttgtacca
     4021 ttatgcttat tcactaggca acaaatatat tttcagacct agaaaagctg caaatgttac
     4081 tgaatacaag tatgtcctct tgtgttttag acatttatga actttccttt atgtaatttt
     4141 ccagaatcct tgtcagattc taatcattgc tttataatta tagttatact catggatttg
     4201 tagttgagta tgaaaatatt ttttaatgca ttttatgact tgccaattgt tcgaacgtac
     4261 gtcgcgaCTC GAGATAACTT CGTATAATGT ATGCTATACG AAGTTATGgc cctactccaa
     4321 aaatgtcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
     4381 aatttcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac
     4441 agtagaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat
     4501 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccncga ggagcatcgt
     4561 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg acatctccac
     4621 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
     4681 aagttcattt catttggaga ggacagccca agcatggaga accgacctgc aggcatgatt
     4741 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4801 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4861 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actccaagac
     4921 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     4981 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     5041 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     5101 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     5161 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     5221 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcggatgcc cgacggcgag
     5281 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     5341 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5401 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5461 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5521 ttcttctgag cgggactctg gggttcggac ggtacgctga aatcaccagt ctctctctac
     5581 aaatctatct ctctctattt tctccataaa taatgtgtga gtagtttccc gataagggaa
     5641 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     5701 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     5761 actaaaatcc agatcAATTC gcggccGATC TATAACTTCG TATAATGTAT GCTATACGAA
     5821 GTTATACTAG tcagaattag attgacatat atgttgaaaa tggatcaaag agaatcttca
     5881 gctaaaaaaa ttatataaat tctatttttg gaccatattg tataaaaaca cttgaaatcc
     5941 tcttgatttc tatttcccta catgggggta tttttctatt tttacaacta ttcaatacat
     6001 actaaaatgc tgaagttatt ggaaatgggt tgtcaacctt gtattacatc cacgatggat
     6061 acttagtgta gagttagaga tctcttactt gatcatatat atatgtatat gtaaatgtgt
     6121 ttcaacaaaa cgagtaccat taaagatgtt tctgacttgg attaagtgct aattccatta
     6181 cagttgatac tgttacatgt caacttccaa ttacatgtgg acgaacatgt ttgacggaaa
     6241 atgtccacac tcacctagat ttcattagtc atccattggt catctaatct gaaacgtcca
     6301 cagcattagt cgaaagacgg aaggaatcaa cgccgacaac aagaaaccag gtgcgagatc
     6361 gaggggtgag ggcggctgca taggtgtcgg tagtgcactc cgttcgtaga gtttagagtt
     6421 tctctgcatt gttgtcaaaa aacagcgagc ctcttgcaaa tggaggtggt gaaatcaatg
     6481 ctgtacacag aaggagacga tcggttgcct ctctattggt acatcgacac cgtggtgatt
     6541 gcattgcgaa gacaagtcta gggtgcatgt gagagacact gggcaagctg gaggcgacaa
     6601 gcgtgaggta ttcacgctcg tggagtcgaa ctggatgcaa tcggggattt acaagcacag
     6661 cttgacagtc aagacggcgg gtatgaagtt tctgatgcaa aattcgtcgc cacgtctaga
     6721 tcatacgcag catgtgcgga ctgtggagca tcctgtacga ttgcgactgg atgttaatat
     6781 tttctttttc cagccaggaa agagtaaact catctgacca agaatggaat gccgaaatcg
     6841 tcacagtcga gcatttgcgg agaaagaacc ctccttgcga atgtgttgtc ccttgttgaa
     6901 atgaatcgta ctgctgagtc atgacgatat acaagagcat ccaactgctt agatctgaca
     6961 ggaagagtaa ttaggtgtgg ggttattgtg gacttagaca tttgtctcaa gttctagtct
     7021 cgcatcacgg aagccggaga aggatgtggc cgatctggcc ctggcgtaat agcgaagagg
     7081 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     7141 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     7201 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7261 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7321 cacgttcgcc ggctttcccc gtcaagctct aaatcggggt gggccatcgc cctgatagac
     7381 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     7441 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     7501 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     7561 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     7621 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     7681 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     7741 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     7801 ggttaatgtc atgggggggg gcacgttgtg tctcaaaatc tctgatgtta cattgcacaa
     7861 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     7921 ggtgttATGA GCCATATTCA ACGGGAAACG TCTTGCTCGA CGAGGCCGCG ATTAAATTCC
     7981 AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT
     8041 GCGACAATCT ACCGATTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC
     8101 AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA
     8161 TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC
     8221 ACCACTGCGA TCCCCGGCAA AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT
     8281 GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT
     8341 AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT
     8401 AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA
     8461 GTCTGGAAAG AAATGCATAA GCTcTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT
     8521 GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT
     8581 GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT
     8641 GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT
     8701 ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAAtcaga attggttaat
     8761 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     8821 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8881 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8941 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9001 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     9061 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9121 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9181 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9241 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     9301 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9361 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9421 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9481 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9541 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9601 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
