LOCUS               5447 bp ds-DNA     circular     09-NOV-2007
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Is there a KpnI site at 23 bp
              CCATCTGTGGGTTAGCGTTC   TAACTTAGACATGCAATGCTC
            ACCATCTGTGGGTTAGCGTCC   TAACTTAGACATGCAATGCTC
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    join(1382..1569,1571..1671,1673..2556)
                     /label=Pp108
                     /ApEinfo_fwdcolor=#008040
                     /ApEinfo_revcolor=#008040
     primer_bind     complement(2092..2111)
                     /label=Pp108-772R
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     misc_feature    join(23..193,195..926,928..959,961..1381)
                     /label=Pp108(1)
                     /ApEinfo_label=Pp108
                     /ApEinfo_fwdcolor=#008040
                     /ApEinfo_revcolor=#008040
     primer_bind     complement(4712..4733)
                     /label=Ubi-970R
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
     primer_bind     5407..5424
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     complement(2604..2624)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     join(5423..5447,1..3)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     complement(2573..2598)
                     /label=SP6
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     rep_origin      complement(2992..3674)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     rep_origin      4822..5262
                     /label=F1 ori
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             complement(5268..5336)
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_revcolor=cornflower blue
     CDS             complement(3772..4431)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
ORIGIN
        1 GGGCGAATTg ggcccattta aaTACCTGTT TAGTTAACAC AAGCAACAAA TTATACTTTC
       61 GCCTATCAGG ACGCCAATTA CAAAGCAACC TAACTAGTAC TAGTCATGCA AGTCAAATCC
      121 TTATCTTCCG ATAGTGTGCA ACCAAATACC GACCCTGCAC CTATCGAAGA CACAGCGATC
      181 ATATCAAATC ACAATTTGAC ATGTCAAGAT GCAAACTTGT TATCTACACA AAATTGACCT
      241 AAGATATAAT TAAGCCTATA GAGCTCCATA TCATAGGCTT ACTCATGAAA ACATCAACTC
      301 ACGGACTGTC GTCTTTCAAG AAGAACGTTA GCATGAATGT TCCACAATAA ACAAAAAGAA
      361 AAGTTTAGAA GTAAGGACAG ATACCATCGA CACTGGAGAA AATAGCCATC CGTAGACGAT
      421 GCCTCGGTAA CATCTTCAGG AGAAAGACTC GAATCTCCCC GTCGCAACAG CACATAGGCC
      481 GACAACTCGC AGCCTACAAT GGGAATGTCG GTCGGGAGGT GCACCCGCAC TACACTCAGC
      541 ATTCTTCCCC GCTTTCGCCC CCTCCCTTAT CTCCAGCTCC GCTTAAGATG ATACTCACGC
      601 TTTTGTACTT CGGCTCTTTC ACTTCCAGAC GAATTTCATG AAAAGTTACA TGTGCTTGAG
      661 GCCGATCAGC CATCAGGCGC CCCCTGTGCG CGCCAGCGTT CTCCCCTCAA CCTCGCCCCC
      721 TCTCACTCTT TGAAAAGTGT GTGTCTAAAT CTGGCAGTGG CGATCCCTCG AAACCTAAAA
      781 CTCTTGCGAA ACATAAGTAA CTTAAGTTTT CCTCTACAAA ACTTGCAAGT ACGACATGCA
      841 CCCACACAAG TGCAACACGG ACGCCTCTTG TTTTCTCTAC ATTCTCATAG TAGAACATGA
      901 GATTCCTCTC AGAAACGCAT TCCCTCCATT CACTTAATCC CCTGTTCCAC GCGTGTCAGG
      961 AGTACTCTTG CAATCATAAA GCGATGGCTT GGTCATTCAA CACTTGGTAG CTTCCTGCAA
     1021 ATAAACCCTA AAGTTGTCAG AGCACTTGAG GAGAAGCTCT TCCCTCACTC CACCTATTCA
     1081 CACTACCTTT CCACTTGAGA AGGACAGCTG CTGCAGCAAA CCCCCAAAAA AAAATAGGGA
     1141 GCGGGAGGGG AACAATAAAT GCATTACCTA AACCACGCAA CCATCTCTCA TCGAACAAGC
     1201 CTCACCGCAA CGCAGACTCT TCCTCCAACA CCCCTCACCA TTCTAACAAG ACTACAAACC
     1261 CATGCTCCAT ACAAGCAACA AAACAACTCT GTTAGAGCGA CGAAATGATC CCACCCAGCC
     1321 TGGTACCAAT TCCTTTATAT TTATGACCTC GTACCACACC ACTTCCAGGC TCAGAAGCAG
     1381 AATCGATTCA GCAGTCACTA ACCCTCTCTA TGCGACAGCC ACGAGAATTA CAAACTGGGT
     1441 CTGCCGGACT TACATATCAA TCAACGGAAT CACGTCCAAG TAAAATAATC CAGCGAACTT
     1501 ATCCACATAG GATCTGAGGG GGGAACGTGA ATGAGCCAGG TCTACACGCA GCATGGGAGA
     1561 GAAGGGGGGG GGGGTTTTCA AATAGAGTCC AAAATTAGCC CAAACGAGGG AGGGAGAATG
     1621 AATGAGGCTG TCGAGGGCGG GCGGAGATGG AACGAAGAGT CGGGGGGGGG GGGGATAGCG
     1681 AAGGCGAAGA GGCTCTCACC CACCCATTGC GCTTCTTTGC GAACTCGACC ACCGAATCTC
     1741 TCCCTCCCTT CCCTCTTCCT CTCCCTCTCC TCTCTCTCTC TCTCTCGTAT GTGTGTGTGT
     1801 GTCTAAATTC GCCCAATTCG TCGTCCCTTC CGCCATCGCC ATTGCCATGC CAATCAATGC
     1861 CTTAGAAACG GGCACTACCA TGGCATCCCC CCCTTTGCAT CCTTGGACCC TCCGGAGGGA
     1921 GGGAGGGAGT CAACGTTCAC ACTCCGAAGT CAACCTGGTC GCTAGACTTT TGGCTTCCTC
     1981 TGTTTATCTT TTGTCTCGGT AGATCCCTCT TGGAATATAT GGATGGATGG ATGAAATTAT
     2041 GGATGAATGA TTGAATGGCA TTTGTGAACC ATTCGATCCC TCCTGAGTGC CTGTGTCCGT
     2101 CAGTCCGTGT AACTGTGACT GACTGTTCTA TCTCATCATC CATCCTTTCT TCATGCTCTA
     2161 CTCGGACTGA ACTCTCTCTC TCTCTCTCTC TCTCTCTCTC GCTCTGTGTA TGTGTGTGTG
     2221 TGTTTGTTTA TTGACTTCTG AACTTGTCAT TTGGTTCTAA TGTAGAGTGA AAGTCGTTTA
     2281 AAGCAGAATA TGCAAATCGT GCATTTTAAG TGGTGTAATG TAATGTATTT TGGGGTGGTG
     2341 ATGTCATGTA CTAAAAAAGG GTAGCACTGA ACTTGTGTCA ATGGTAGTCA GATAACAATA
     2401 GATTTTCGAA ATGTATCCAA GTGTTGACTT TGAAAACTGA CTTGGGAAAT GAAATGTGAT
     2461 ATTTTCAATG GTATTTAGTT TTAAGTTATT CATTAATAGT GTATTTTTAG ATGCAGTGAA
     2521 ATGTTTTGTT AGATAAAACT CTAATCAAAT TTAAATgcat AGCTTGAGTA TTCTATAGTG
     2581 TCACCTAAAT AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC
     2641 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA
     2701 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA
     2761 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT
     2821 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG
     2881 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC
     2941 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT
     3001 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     3061 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     3121 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     3181 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     3241 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     3301 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     3361 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     3421 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     3481 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     3541 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     3601 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     3661 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     3721 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
     3781 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
     3841 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
     3901 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
     3961 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
     4021 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
     4081 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
     4141 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
     4201 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
     4261 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
     4321 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
     4381 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
     4441 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
     4501 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
     4561 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
     4621 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT
     4681 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT
     4741 TCCCCGAAAA GTGCCACCTG ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT
     4801 ACCGCATCAG GAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA
     4861 AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA
     4921 ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA
     4981 CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA
     5041 ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC
     5101 TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA
     5161 AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG
     5221 CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCCA TTCGCCATTC
     5281 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG
     5341 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA
     5401 CGACGTTGTA AAACGACGGC CAGTGAATTG TAATACGACT CACTATA 
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