LOCUS               4327 bp ds-DNA     circular     26-OCT-2007
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     lox-35S-hygro-cter-lox prim (a) fw:m13F prim (b):m13rev
            Hyg has false ATG and no stop!!!
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        324..737
                     /label=35S Promoter
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
     CDS             complement(2..70)
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_revcolor=cornflower blue
     primer_bind     141..158
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     complement(1715..1735)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     complement(1685..1704)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     rep_origin      complement(2103..2785)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             complement(2883..3542)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     misc_recomb     complement(282..315)
                     /label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
     misc_recomb     complement(1579..1612)
                     /label=LoxP(1)
                     /ApEinfo_label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
     primer_bind     689..708
                     /label=35Stata-F
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     terminator      1397..1555
                     /label=Tcamv
                     /ApEinfo_fwdcolor=#800000
                     /ApEinfo_revcolor=#800000
     CDS             807..1340
                     /label=Gentamicin Resistance
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#cc66ff
ORIGIN
        1 CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC
       61 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG
      121 GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGC Caagctaatt accctgttat
      181 ccctaggcgg accgaagctt gcatgcctgc aggtcgactc tagaggatcc cacgtgcccg
      241 ggcgtttaaa ccaattgttc gaacgtacgt cgcgaCTCGA GATAACTTCG TATAATGTAT
      301 GCTATACGAA GTTATggtac caacatggtg gagcacgaca ctctcgtcta ctccaagaat
      361 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
      481 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
      661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
      721 tcatttcatt tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc
      781 tcgattcgca gatctgtcga tcgaccATGG TACGCAGCAG CAACGATGTT ACGCAGCAGG
      841 GCAGTCGCCC TAAAACAAAG TTAGGTGGCT CAAGTATGGG CATCATTCGC ACATGTAGGC
      901 TCGGCCCTGA CCAAGTCAAA TCCATGCGGG CTGCTCTTGA TCTTTTCGGT CGTGAGTTCG
      961 GAGACGTAGC CACCTACTCC CAACATCAGC CGGACTCCGA TTACCTCGGG AACTTGCTCC
     1021 GTAGTAAGAC ATTCATCGCG CTTGCTGCCT TCGACCAAGA AGCGGTTGTT GGCGCTCTCG
     1081 CGGCTTACGT TCTGCCCAAG TTTGAGCAGC CGCGTAGTGA GATCTATATC TATGATCTCG
     1141 CAGTCTCCGG CGAGCACCGG AGGCAGGGCA TTGCCACCGC GCTCATCAAT CTCCTCAAGC
     1201 ATGAGGCCAA CGCGCTTGGT GCTTATGTGA TCTACGTGCA AGCAGATTAC GGTGACGATC
     1261 CCGCAGTGGC TCTCTATACA AAGTTGGGCA TACGGGAAGA AGTGATGCAC TTTGATATCG
     1321 ACCCAAGTAC CGCCACCTAA CAATTCGTTC AAGCCGAGAT CGGCTTCCCG GCCTAGAGTC
     1381 GATCGACAAG CTCGAGTTTC TCCATAATAA TGTGTGAGTA GTTCCCAGAT AAGGGAATTA
     1441 GGGTTCCTAT AGGGTTTCGC TCATGTGTTG AGCATATAAG AAACCCTTAG TATGTATTTG
     1501 TATTTGTAAA ATACTTCTAT CAATAAAATT TCTAATTCCT AAAACCAAAA TCCAGGAGCT
     1561 CGAATTCgcg gccGATCTAT AACTTCGTAT AATGTATGCT ATACGAAGTT ATACTAGTgg
     1621 cgccacgcgt gatatcatgc atgttaacat cgatccatgg gcgcgcctta attaaattta
     1681 aattCCCTAT AGTGAGTCGT ATTAAATTCG TAATCATGGT CATAGCTGTT TCCTGTGTGA
     1741 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC
     1801 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC
     1861 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC
     1921 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT
     1981 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA
     2041 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA
     2101 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
     2161 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
     2221 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC
     2281 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT
     2341 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC
     2401 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG
     2461 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA
     2521 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC
     2581 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA
     2641 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA
     2701 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     2761 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     2821 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     2881 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     2941 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     3001 AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     3061 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     3121 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     3181 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     3241 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     3301 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     3361 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     3421 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     3481 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     3541 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     3601 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     3661 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     3721 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     3781 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     3841 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG
     3901 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT
     3961 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA
     4021 AATCGGGGCA TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA
     4081 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT
     4141 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC
     4201 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG
     4261 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT
     4321 ACAATTT     
//
