LOCUS               5136 bp ds-DNA     circular     09-NOV-2007
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     lox-35S-hygro-cter-lox prim (a) fw:m13F prim (b):m13rev
            Hyg has false ATG and no stop!!!
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     141..158
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     complement(2524..2544)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     primer_bind     complement(2494..2513)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     rep_origin      complement(2912..3594)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             complement(2..70)
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_revcolor=cornflower blue
     CDS             complement(3692..4351)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     misc_recomb     complement(282..315)
                     /label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
     misc_recomb     complement(2388..2421)
                     /label=LoxP(1)
                     /ApEinfo_label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
     promoter        join(325..1023,1025..1104)
                     /label=35S
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
     primer_bind     647..664
                     /label=P35S-KpnF
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     primer_bind     complement(2340..2364)
                     /label=T35S-SacI
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     primer_bind     complement(2046..2065)
                     /label=hptII-R
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     primer_bind     1852..1871
                     /label=hptII-F
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     primer_bind     2089..2108
                     /label=HYG-F
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     gene            1140..2165
                     /label=HygR
                     /ApEinfo_fwdcolor=#00ff80
                     /ApEinfo_revcolor=#00ff80
     primer_bind     1014..1033
                     /label=35Stata-F
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     terminator      2206..2364
                     /label=Tcamv
                     /ApEinfo_fwdcolor=#800000
                     /ApEinfo_revcolor=#800000
ORIGIN
        1 CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC
       61 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG
      121 GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGC Caagctaatt accctgttat
      181 ccctaggcgg accgaagctt gcatgcctgc aggtcgactc tagaggatcc cacgtgcccg
      241 ggcgtttaaa ccaattgttc gaacgtacgt cgcgaCTCGA GATAACTTCG TATAATGTAT
      301 GCTATACGAA GTTATGGTAc caacatggtg gagcacgaca ctctcgtcta ctccaagaat
      361 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
      421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
      481 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgaaca tggtggagca
      661 cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat
      721 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
      781 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      841 cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc
      901 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      961 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     1021 agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct gaaatcacca
     1081 gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg caatgagata
     1141 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     1201 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     1261 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     1321 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     1381 gggagtttag cgagagcctg acctattgca tctcccgccg ttcacagggt gtcacgttgc
     1441 aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag gctatggatg
     1501 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     1561 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     1621 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     1681 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     1741 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     1801 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     1861 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccac
     1921 gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     1981 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     2041 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     2101 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaaga
     2161 aatagagtag atgccgaccg ggatctgtcg atcgacaagc tcgagtttct ccataataat
     2221 gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga
     2281 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     2341 ctaattccta aaaccaaaat ccagGAGCTC GAATTCgcgg ccGATCTATA ACTTCGTATA
     2401 ATGTATGCTA TACGAAGTTA TACTAGTggc gccacgcgtg atatcatgca tgttaacatc
     2461 gatccatggg cgcgccttaa ttaaatttaa attCCCTATA GTGAGTCGTA TTAAATTCGT
     2521 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
     2581 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT
     2641 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT
     2701 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT
     2761 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA
     2821 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA
     2881 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC
     2941 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA
     3001 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC
     3061 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT
     3121 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT
     3181 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG
     3241 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA
     3301 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT
     3361 ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA
     3421 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT
     3481 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA
     3541 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT
     3601 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA
     3661 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT
     3721 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA
     3781 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT
     3841 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG
     3901 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA
     3961 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT
     4021 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA
     4081 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA
     4141 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA
     4201 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT
     4261 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG
     4321 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC
     4381 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT
     4441 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA
     4501 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT
     4561 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT
     4621 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG
     4681 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG
     4741 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA
     4801 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT CCCTTTAGGG TTCCGATTTA
     4861 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC
     4921 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG
     4981 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT
     5041 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA
     5101 ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTT  
//
