LOCUS                pLoxNPTII        4547 bp ds-DNA   circular     16-DEC-2008
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     pMBL5's nptII (BglII/T4P/KpnI) into pB-Lox (EcoRI/T4P/KpnI)
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     141..158
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     promoter        join(319..553,555..709)
                     /note="Cauliflower Mosaic Virus 35S promoter"
                     /label=CaMV 35S promoter
                     /ApEinfo_fwdcolor=#008100
                     /ApEinfo_revcolor=#008100
     primer_bind     complement(1935..1955)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     gene            742..1536
                     /gene="nptII"
                     /label=nptII
                     /ApEinfo_fwdcolor=#ccff66
                     /ApEinfo_revcolor=#ccff66
     primer_bind     complement(1905..1924)
                     /label=T7
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     rep_origin      complement(2323..3005)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     terminator      join(1562..1581,1583..1767)
                     /label=Tcamv
                     /ApEinfo_fwdcolor=#ff6666
                     /ApEinfo_revcolor=#ff6666
     CDS             complement(2..70)
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=#8000ff
                     /ApEinfo_revcolor=#8000ff
     CDS             complement(3103..3762)
                     /label=AmpR
                     /ApEinfo_fwdcolor=#8000ff
                     /ApEinfo_revcolor=#8000ff
     misc_recomb     complement(282..315)
                     /label=LoxP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_recomb     complement(1799..1832)
                     /label=LoxP(1)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     primer_bind     660..679
                     /label=35Stata-F
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     primer_bind     743..764
                     /label=KANR
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
     primer_bind     complement(1506..1527)
                     /label=KANF
                     /ApEinfo_fwdcolor=#66ffff
                     /ApEinfo_revcolor=green
ORIGIN
        1 CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC
       61 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG
      121 GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGC Caagctaatt accctgttat
      181 ccctaggcgg accgaagctt gcatgcctgc aggtcgactc tagaggatcc cacgtgcccg
      241 ggcgtttaaa ccaattgttc gaacgtacgt cgcgaCTCGA GATAACTTCG TATAATGTAT
      301 GCTATACGAA GTTATGccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag
      361 ggctattgag acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc
      421 agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca
      481 tcattgcgat aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga
      541 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      601 gcaagtggat tgatgtgaca tctccactga cgtaagggat gacgcacaat cccactatcc
      661 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cagcccaagc
      721 atggagaacc gacctgcagg catgattgaa caagatggat tgcacgcagg ttctccggcc
      781 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
      841 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
      901 tccggtgccc tgaatgaact ccaagacgag gcagcgcggc tatcgtggct ggccacgacg
      961 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     1021 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     1081 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     1141 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     1201 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     1261 ctcaaggcgc ggatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     1321 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     1381 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     1441 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     1501 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcggacggt
     1561 acgctgaaat caccagtctc tctctacaaa tctatctctc tctattttct ccataaataa
     1621 tgtgtgagta gtttcccgat aagggaaatt agggttctta tagggtttcg ctcatgtgtt
     1681 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     1741 ttctaattcc taaaaccaaa atccagtact aaaatccaga tcAATTCgcg gccGATCTAT
     1801 AACTTCGTAT AATGTATGCT ATACGAAGTT ATACTAGTgg cgccacgcgt gatatcatgc
     1861 atgttaacat cgatccatgg gcgcgcctta attaaattta aattCCCTAT AGTGAGTCGT
     1921 ATTAAATTCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT
     1981 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG
     2041 CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG
     2101 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC
     2161 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC
     2221 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA
     2281 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
     2341 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     2401 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     2461 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     2521 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     2581 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     2641 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     2701 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     2761 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     2821 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     2881 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
     2941 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     3001 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     3061 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     3121 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     3181 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     3241 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     3301 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     3361 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     3421 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     3481 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     3541 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     3601 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     3661 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     3721 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     3781 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     3841 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     3901 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     3961 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     4021 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
     4081 AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG
     4141 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC
     4201 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGCA TCCCTTTAGG
     4261 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC
     4321 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
     4381 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC
     4441 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA
     4501 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTT 
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