Cloning

Amplification of cDNA
Competent Cells
Cloning general - ligations, digests
Mini Preps - Dirty

Tissue Culture

Protein CoIPs
FLAG-protein-RNA-coIP
Frozen stocks of Tissue Culture Cells
Immunofluorescence Microscopy
In Situ Hybridization
Polysome Profiling by Sucrose Gradient
Preparation of Total TC RNA
Pulse Chase mRNA Decay Assay
siRNA transfection for HeLa and Tet-off cells
Tandem siRNA transfection
Puromycin Selection

Protein Work

Purification of GST Tagged Protein
Affinity purification of rabbit anti-GST-tagged antigen serum
Purification of His-tagged protein
SDS-PAGE Gels
Western Blot
2D Gel

In Vitro RNA

Capping, mRNA
Decapping Assay
In Vitro Transcription
Northern Blot
Northern Blot, Polyacrylamide
Poly A RNA Preparation
Preparation of Riboprobe

Ng108 Neuronal Cells

Freezing Ng108s
Immunocytochemistry of Ng108s

Drosophila S2 Cells

Freezing S2 Cells
Fixing S2 onto Coverslips
RNAi in S2 Cells