Hypotonic gentle lysis buffer:
10 mM Tris-HCl pH 7.5 10 mM NaCl 10 mM EDTA 0.5% Triton-X100
Formamide load buffer:
Deionized Formamide 5 mM EDTA 0.1% Bromophenol blue 0.1% Xylene Cyanol