Spin down 10x ConA stock and dilute to 1x (500µg/ml) in PBS
Spin down 1x stock
Add 250 µl of 1x ConA in PBS to a clean cover slip in 12-well plate
Incubate for 30min.
Wash cover slip with 500µl PBS
Repeat 2x
Transfer cover slip to new 12-well plate
Add ~100k cells to cover slip
Spin @ 0.5k RPM for 1min.
Incubate for 1h @ RT
Wash cover slip gently with 1ml PBS
Add 500µl 4% paraformaldehyde to cover slip Incubate 15min.
Add 500µl 0.01% TX-100 in PBS
Wash w/ 1ml PBS
Store @ 4°C or stain immediately