From 3.5 cm plate:

Prepare total RNA using Trizol.
Dissolve RNA in 80 µl ddw.

Annealing:
Add 0.5 µl Biotinylated oligo-dT probe
Add 2 µl 20xSSC
Heat RNA at 65C, 10 min.
Incubate at RT, 10 min. (do not place on ice)

For each RNA purification prepare:
350 µl 0.5xSSC
450 µl 0.1xSSC

Washing magnetic beads:
Resuspend magnetic beads by pipetting up and down (no spin!)
For each RNA prep, transfer 95 µl beads to eppendorf tube.
Transfer tube to magnetic stand. Remove supernatant carefully.
Wash beads 3 times with 100 µl 0.5xSSC (every wash: remove tube from stand and resuspend beads by pipetting up and down).
Resuspend beads in 20 µl 0.5xSSC

Capturing polyA mRNA:
Add entire annealed reaction to the washed beads.
Incubate at RT, 10 min, in vortexer at 1,500 rpm to keep beads resuspended.
Transfer tube to magnetic stand. Remove supernatant carefully.
Wash beads 4 times with 100 µl 1xSSC (every wash: remove tube from stand and resuspend beads by pipetting up and down).

Elution:
Resuspend beads in 50 µl ddw.
Capture beads and transfer supernatant (containing polyA RNA) to new tube.
Repeat elution and combine with previous eluate (giving 100 µl total).
Add 10 µl 3M NaAc (pH 6.0)
Add 1 µl glycogen.
Add 275 µl 100% EtOH
ppt., wash, dry
Dissolve in 20 µl Formamide LB