Small mighty tall gels:

Separation gel

10 ml total: (~6mL/gel)
x ml ddw
x ml 40% acrylamide mix (37.5:1)
3.75 ml 1M Tris-HCl pH 8.8
0.1 ml 10% SDS
10 µl Temed
100 µl 10% APS

Pour gel, leave appr. one inch space for stacking.
Overlay with 0.5 ml water or EtOH.
After polymerization, rinse gel with water.

Stacking gel
10 ml total: (~2.5mL/gel)
7.0 ml ddw
1.25 ml 40% acrylamide mix (37.5:1)
1.25 ml 1M Tris-HCl pH 6.8
0.1 ml 10% SDS
10 µl TEMED
100 µl 10% APS

Pour stacking gel and insert comb.
Wait one hour till use for best results.
Denature protein samples with caps on epi lids at 95C 1-2 min (not marker!) or 5-10 min for whole cell lysates.
Set up with 1xSDS running buffer and load samples.
Run at 130V through stacking and 230V through separation.