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The AGL15 MADS-box gene is highly expressed in developing embryos, specifically in the embryo proper. We did not detect AGL15 RNA in the suspensor cells. 


Gene Sequence

Map position

We first detected AGL15 RNA (yellow) at the 8-cell stage of embryo development, as shown below.

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The next images show the embryo at the dermatogen stage. Notice the strong uniform accumulation of AGL15 RNA throughout the embryo, and the lack of expression in the suspensor.

agl15_derm_bf.gif (32273 bytes)agl15_derm_df.gif (20694 bytes)

This uniform expression of AGL15 RNA is maintained throughout embryo development and seedling germination.

Selected References:

Heck, G.R., Perry, S.E, Nichols, K.W., and Fernandez, D. (1995). AGL15, a MADS-domain protein expressed in developing embryos. Plant Cell 7:1271-1282.

Fernandez D.E., Heck G.R., Perry S.E., Patterson S.E., Bleecker A.B, Fang S.C. (2000) The embryo MADS domain factor AGL15 acts postembryonically. Inhibition of perianth senescence and abscission via constitutive expression. Plant Cell 12:183-98

Perry, S.E., Nichols, K.W., and Fernandez, D.E. (1996). The MADS-domain protein AGL15 localizes to the nucleus during early stages of seed development. Plant Cell 8:1977-1989.

Rounsley, S.D., Ditta, G.S , and Yanofsky, M.F. (1995). Diverse roles for MADS box genes in Arabidopsis development. Plant Cell 7:1259-1269.