pMP series vectors

Expression vectors with Gateway

pMP1155 (pUK-Pp108B-nptII-Ubi:Gateway:ocs)                           Map Seq

pMP1228 (pUK-Pp108B-nptII-Ubix:Gateway:ocs)(no intron)        Map Seq

pMP1180 (pUK-Pp108B-nptII-GmHsp17.3B:Gateway:e9)            Map Seq

RNAi vectors with Gateway

pMP1331 (pBiLoxHyg+Ubix:Hellsgate12:ocs)                              Map Seq  

pMP1333 (pUK-Pp108B-Hyg+Ubix:Hellsgate12:ocs)                   Map Seq  

pMP882   (pENTR-NotIBamHISmaIEcoRI)                                    Map Seq

Physco selectable marker plasmids  (derived from pBNRF)

pMP1109 (pBiLox+35S:nptII:camv) G418/Kanamycin-res.           Map Seq  

pMP1159 (pBiLox+2x35S:Hyg:camv) Hygromycin-res.                 Map Seq  

pMP1169 (pBiLox+35S:Zeo:camv) Zeocin-res.                             Map Seq  

pMP1119 (pBiLox+35S:Gent:camv) Gentamycin-res.                   Map Seq

Expression vectors with Gateway and tags

pMP1300 (pUK-Pp108B-nptII-Ubi:Gateway:GUS:ocs)                  Map Seq  

pMP1367 (pUK-Pp108B-nptII-Ubi:Gateway:TAP:ocs)                   Map Seq  

pMP1302 (pUK-Pp108B-nptII-Ubi:Gateway:TAP2:ocs)                 Map Seq   

pMP1298 (pUK-Pp108B-nptII-Ubi:Gateway:GFPhdel:ocs)           Map Seq  

pMP1382 (pUK-Pp108B-nptII-Ubi:Gateway:GFP-His:ocs)            Map Seq  

pMP1379 (pUK-Pp108B-nptII-Ubi:Gateway:YFP-HA:ocs)             Map Seq  

pMP1574 (pUK-Pp108B-nptII-Ubi:Gateway:CFP-HA:ocs)            Map Seq  

pMP1472 (pUK-Pp108B-nptII-Ubi:Gateway:YFPnls-HA:ocs)       Map Seq  

pMP1442 (pUK-Pp108B-nptII-Ubi:Gateway:mVenus-HA:ocs)      Map Seq  

pMP1474 (pUK-Pp108B-nptII-Ubi:Gateway:mVenusnls-HA:ocs)Map Seq  

pMP1445 (pUK-Pp108B-nptII-Ubi:Gateway:DsRED2-HA:ocs)     Map Seq  

pMP1475 (pUK-Pp108B-nptII-Ubi:Gateway:DsRED2nls-HA:ocs)Map Seq  

pMP1377 (pUK-Pp108B-nptII-Ubi:Gateway:4xMyc:nos)               Map Seq  

pMP1373 (pUK-Pp108B-nptII-Ubi:Gateway:1xMyc:ocs)               Map Seq  

pMP1369 (pUK-Pp108B-nptII-Ubi:Gateway:HA:ocs)                     Map Seq  

pMP1371 (pUK-Pp108B-nptII-Ubi:Gateway:FLAG:ocs)                Map Seq  

pMP1375 (pUK-Pp108B-nptII-Ubi:Gateway:AcV5:ocs)                 Map Seq  

pMP1575 (pUK-Pp108B-nptII-Ubi:Gateway:GRlbd:ocs)                 Map Seq  

pMP1578 (pUK-Pp108B-nptII-Ubi:Gateway:ESR1lbd:ocs)             Map Seq  

pMP1335 (pUK-Pp108B-nptII-Ubi:GFP6:Gateway:ocs)                 Map Seq  

pMP1361 (pUK-Pp108B-nptII-Ubi: YFP:Gateway:ocs)                  Map Seq  

pMP1363 (pUK-Pp108B-nptII-Ubi:TAP2:Gateway:ocs)                 Map Seq  

pMP1359 (pUK-Pp108B-nptII-Ubi:1xMyc:Gateway:ocs)               Map Seq  

pMP1355 (pUK-Pp108B-nptII-Ubi:HA:Gateway:ocs)                     Map Seq  

pMP1357 (pUK-Pp108B-nptII-Ubi:FLAG:Gateway:ocs)                Map Seq  

pMP1365 (pUK-Pp108B-nptII-Ubi:AcV5:Gateway:ocs)                 Map Seq

Physco neutral target loci plasmids

pMP1141 (pGEM+Pp108A) hydrolase pseudogene target           Map Text    

pMP1033 (pUK+Pp108B) adjacent intergenic region target          Map Text  

Pp108 target locus                                                                         Map Text

Protocols

Moss Transformation.pdf

E. coli Ultra comp cells

Sequence editor

ApE  -  A Plasmid Editor